> For the complete documentation index, see [llms.txt](https://edu.abi.am/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://edu.abi.am/ngs-data-analysis-and-functional-genomics/experimental-techniques/the-third-generation-of-sequencing-technologies.md). # The third generation of sequencing technologies The third generation of sequencing technologies has enabled the generation of long stretches of contiguous sequence information from a single molecule of DNA. This can be useful for resolving complex genomic structures and accurately assembling large genomes. Additionally, third-generation sequencing technologies may enable fast and cost-efficient applications, for example, in diagnostics. The common technologies are Single Molecule Real-Time (SMRT) sequencing by Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) sequencing. Watch the video below to understand how Nanopore sequencing works. {% embed url="" %} ##