ABI Bioinformatics Guide 2024
  • INTRODUCTION
    • How to use the guide
  • MOLECULAR BIOLOGY
    • The Cell
      • Cells and Their Organelles
      • Cell Specialisation
      • Quiz 1
    • Biological Molecules
      • Carbohydrates
      • Lipids
      • Nucleic Acids (DNA and RNA)
      • Quiz 2
      • Proteins
      • Catalysis of Biological Reactions
      • Quiz 3
    • Information Flow in the Cell
      • DNA Replication
      • Gene Expression: Transcription
      • Gene Expression: RNA Processing
      • Quiz 4
      • Chromatin and Chromosomes
      • Regulation of Gene Expression
      • Quiz 5
      • The Genetic Code
      • Gene Expression: Translation
    • Cell Cycle and Cell Division
      • Quiz 6
    • Mutations and Variations
      • Point mutations
      • Genotype-Phenotype Interactions
      • Quiz 7
  • PROGRAMMING
    • Python for Genomics
    • R programming (optional)
  • STATISTICS: THEORY
    • Introduction to Probability
      • Conditional Probability
      • Independent Events
    • Random Variables
      • Independent, Dependent and Controlled Variables
    • Data distribution PMF, PDF, CDF
    • Mean, Variance of a Random Variable
    • Some Common Distributions
    • Exploratory Statistics: Mean, Median, Quantiles, Variance/SD
    • Data Visualization
    • Confidence Intervals
    • Comparison tests, p-value, z-score
    • Multiple test correction: Bonferroni, FDR
    • Regression & Correlation
    • Dimentionality Reduction
      • PCA (Principal Component Analysis)
      • t-SNE (t-Distributed Stochastic Neighbor Embedding)
      • UMAP (Uniform Manifold Approximation and Projection)
    • QUIZ
  • STATISTICS & PROGRAMMING
  • BIOINFORMATICS ALGORITHMS
    • Introduction
    • DNA strings and sequencing file formats
    • Read alignment: exact matching
    • Indexing before alignment
    • Read alignment: approximate matching
    • Global and local alignment
  • NGS DATA ANALYSIS & FUNCTIONAL GENOMICS
    • Experimental Techniques
      • Polymerase Chain Reaction
      • Sanger (first generation) Sequencing Technologies
      • Next (second) Generation Sequencing technologies
      • The third generation of sequencing technologies
    • The Linux Command-line
      • Connecting to the Server
      • The Linux Command-Line For Beginners
      • The Bash Terminal
    • File formats, alignment, and genomic features
      • FASTA & FASTQ file formats
      • Basic Unix Commands for Genomics
      • Sequences and Genomic Features Part 1
      • Sequences and Genomic Features Part 2: SAMtools
      • Sequences and Genomic Features Part 3: BEDtools
    • Genetic variations & variant calling
      • Genomic Variations
      • Alignment and variant detection: Practical
      • Integrative Genomics Viewer
      • Variant Calling with GATK
    • RNA Sequencing & Gene expression
      • Gene expression and how we measure it
      • Gene expression quantification and normalization
      • Explorative analysis of gene expression
      • Differential expression analysis with DESeq2
      • Functional enrichment analysis
    • Single-cell Sequencing and Data Analysis
      • scRNA-seq Data Analysis Workflow
      • scRNA-seq Data Visualization Methods
  • FINAL REMARKS
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  1. MOLECULAR BIOLOGY
  2. The Cell

Cells and Their Organelles

PreviousThe CellNextCell Specialisation

Last updated 11 months ago

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When using a microscope to magnify a section of tissue from any organism, you will observe that it is composed of units known as cells. The cell is a basic structural and functional unit of an organism.

Multicellular organisms, such as animals and plants, exhibit a complex organisation. For instance, the human body is comprised of approximately 30 trillion cells. In contrast, some organisms, like amoebae, consist of a single cell. Cells come in various shapes and sizes, each equipped with specialised functions that contribute to the overall functioning of living systems. Different functions of a cell can be assigned to specialised compartments called organelles.

Cells can be categorised as eukaryotic or prokaryotic. Two large groups of organisms, bacteria and archaea have prokaryotic cells, while all other organisms have eukaryotic cells. Take a look at the diagram below and try to identify two major similarities and one difference between prokaryotic and eukaryotic cells.

All cells share common characteristics, one of the most notable being the presence of a plasma membrane and ribosomes. The cell membrane serves to control what goes in and out of the cell. The ribosomes perform the synthesis of proteins.

The major difference is the presence of a nucleus and other membrane-enclosed organelles in eukaryotic cells. Membranes separate such organelles from the rest of the cell in order to maintain conditions (e.g. pH or concentration of some molecules) optimised to perform particular tasks. A jelly-like substance surrounding the organelles is called cytosol.

The functions of major organelles that can be found in most eukaryotic cells are described in the table below.

Organelle
Function

Nucleus

Stores most of the cell’s genetic material

Mitochondrion

The site of aerobic respiration, the process that produces adenosine triphosphate (ATP) – a molecule that cells use as a major source of energy

Ribosome

Makes proteins

Rough endoplasmic reticulum

Is covered by ribosomes. Transports proteins to other parts of the cell

Smooth endoplasmic reticulum

Makes lipids, stores calcium ions, breaks toxins

Golgi apparatus

Modifies proteins and packages them into vesicles

Lysosome

Digests molecules

Cytoskeleton

A network of protein filaments that defines the cell shape, enables the cell movement and intracellular transport

Plant cells have some organelles distinguishing them from animal cells.

Plant cell organelle
Function

Permanent vacuole

Contains the cell sap which stores degradative enzymes, waste products and inorganic ions

Chloroplast

Glucose is synthesised here in course of photosynthesis

Cell wall

A rigid structure surrounding the cell which protects the cell and defines its shape

Prokaryotic cells have no nucleus and other membrane-bound organelles. Many prokaryotes are surrounded by two membranes (gram-negative bacteria).

Bacterial cell organelle
Function

Nucleoid

A region where the bacterial chromosome is located

Plasmid

Small circular DNA that often carries genes for antibiotic resistance

Flagellum

An organelle responsible for movement

Summary

The video below provides a summary of the topic and some additional details:

Bacterial (prokaryotic) cell on the left and animal (eukaryotic) cell on the right. Image source: Biorender.com
Animal cell Image source: Biorender.com
Plant cell Image source: Biorender.com
Bacterial cell Image source: Biorender.com
Cells are visible in the epithelium tissue of the mammalian gut (left) and onion root hair tip (right) Image sources (from left to right): Berkshire Community College Bioscience Image Library - Epithelial Tissues: Mucous Glands in Simple Columnar Epithelium, CC0, Johnathan Gutierrez, CC BY-SA 4.0,
Some types of mammalian cells Image sources (from left to right): UC Regents Davis campus - http://brainmaps.org, CC BY 3.0, Electron Microscopy Facility at The National Cancer Institute at Frederick (NCI-Frederick) - [1], Public Domain, CC BY-SA 3.0, SubtleGuest at English Wikipedia, CC BY 2.5,
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