> For the complete documentation index, see [llms.txt](https://edu.abi.am/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://edu.abi.am/ngs-data-analysis-and-functional-genomics/experimental-techniques/sanger-first-generation-sequencing-technologies.md).

# Sanger (first generation) Sequencing Technologies

**DNA Sequencing** is a way to figure out the order of nucleotides along a strand of DNA. **Sanger sequencing** represents the first generation of sequencing technologies, and is based on the process of **DNA replication**. Scientists make copies of DNA strands. Then they observe which nucleotides are added. This way the sequence of nucleotides can be seen. 

To learn more about this topic, navigate through [this link](https://letstalkscience.ca/educational-resources/backgrounders/sanger-sequencing), and completely review the content on the page. Return here once you finish.<br>

You can also watch this video, to better understand the concept of Sanger sequencing

{% embed url="<https://www.youtube.com/watch?v=-QIMkQ4E_wE>" %}

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