> For the complete documentation index, see [llms.txt](https://edu.abi.am/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://edu.abi.am/ngs-data-analysis-and-functional-genomics/experimental-techniques/next-second-generation-sequencing-technologies.md).

# Next (second) Generation Sequencing technologies

Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers ultra-high throughput, scalability, and speed. The technology is used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA. NGS has revolutionized the biological sciences, allowing labs to perform a wide variety of applications and study biological systems at a level never before possible. 

The third generation of sequencing technologies followed, with the power of sequencing long stretches of nucleic acids.

Let's start with the review of 2nd and 3rd Generation Sequencing technologies. 

Now, watch the following video explaining Illumina sequencing by synthesis in more detail.

{% embed url="<https://www.youtube.com/watch?v=mI0Fo9kaWqo>" %}


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