Next (second) Generation Sequencing technologies

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Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers ultra-high throughput, scalability, and speed. The technology is used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA. NGS has revolutionized the biological sciences, allowing labs to perform a wide variety of applications and study biological systems at a level never before possible.

The third generation of sequencing technologies followed, with the power of sequencing long stretches of nucleic acids.

Let's start with the review of 2nd and 3rd Generation Sequencing technologies. Enroll in this Coursera course and watch this video․

Now, watch the following video explaining Illumina sequencing by synthesis in more detail.

Finally, go through these slides to get a better understanding of the three generations of sequencing technologies and the NGS workflow.

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